Aliases cel_circ_new_0005550 Scientific name Caenorhabditis elegans
Organism Nematode Genome Locus chrV:10248906-10250676:+
CircRNA type n/a Gene ID n/a
Gene Name akt-1 Detection pipeline n/a
Sample Type L4 larval stage
Method for Estimation Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer TTCTTGTGAAGCCATCCCTC
Reverse Primer GAGAGCACATTCGAAACTGGA
Pubmed 29298683
Aliases cel_circ_new_0005550 Scientific name Caenorhabditis elegans
Organism Nematode Genome Locus chrV:10248906-10250676:+
CircRNA type n/a Gene ID WBGene00000102
Gene Name akt-1 Detection pipeline find_circ
Sample Type L4 larval stage
Method for Estimation n/a
Circular RNA status Experimented PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer n/a
Reverse Primer n/a
Pubmed 29298683
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
190 GCGAAGAAGTTGCTCACACT TCTTGTGAAGCCATCCCTCA 50 50 58.779 58.639 132 321 20 20
128 CTCGCGAAGAAGTTGCTCAC ATCGACATTCTTTCACCGGC 55 50 59.563 58.63 129 256 20 20
134 TGCAAGCATCCGTTCCTTAC GCCATCCCTCAATGACAACA 50 50 58.551 58.162 179 312 20 20
195 TCATTGCTCGCGAAGAAGTT GTGAAGCCATCCCTCAATGAC 45 52.381 58.204 58.98 123 317 20 21
111 TTGCTCGCGAAGAAGTTGCT GCCGTCGTAAACCTTTCGG 50 57.895 60.88 59.217 126 236 20 19
220 TGTAATCATTGCTCGCGAAGA CGAATGTGCTCTCCTTTCTTGT 42.857 45.455 58.113 58.93 118 337 21 22
113 AGTTGCTCACACTTTGACAGA CATTCTTTCACCGGCGCC 42.857 61.111 57.733 59.515 139 251 21 18
265 AGGAAAAGCGTACTCAAAAGCT TGTGCTCTCCTTTCTTGTGAA 40.909 42.857 58.522 57.441 69 333 22 21