Aliases hsa_circ_0016347 Scientific name Homo sapiens (hg19)
Organism Human Genome Locus chr1:211092981-211192598:-
CircRNA type . Gene ID ENSG00000234233.1
Gene Name KCNH1-IT1 Detection pipeline .
Sample Type A549
Method for Estimation RNA-Seq with Rnase R resistance
Circular RNA status Experimented PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer .
Reverse Primer .
Pubmed 25234927
Aliases Hsa_circ_0016347 Scientific name Homo sapiens (hg19)
Organism Human Genome Locus chr1:211092981-211192598:-
CircRNA type N/A Gene ID ENSG00000234233.1
Gene Name KCNH1-IT1 Detection pipeline N/A
Sample Type Osteosarcoma
Method for Estimation Microarray and Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer ACACGTCTTGCCCTCATTATCT
Reverse Primer ATAACCTTGGGCTTGTCTTTCA
Pubmed 28424426
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
150 GATTGTCTCGGTGGGTTGAT GTCTCCTTCAAAACCAGGCA 50 50 57.597 58.019 94 243 20 20
130 TGTCTCGGTGGGTTGATTCG GCAGAATAATGTGGCCTGGC 55 55 60.038 59.613 97 226 20 20
282 GTGGGTTGATTCGGCACAAA CTCAGACATCCTTCCCCAGT 50 55 59.329 58.422 104 385 20 20
234 TGTGGAAGGGGAAGCACTC CAGACATCCTTCCCCAGTACA 57.895 52.381 59.239 58.812 150 383 19 21
240 CTTCCCAGCTTCCCGTCC CGTGGGATTGGATCATCTTGA 66.667 47.619 59.731 57.802 63 302 18 21
139 GGTGGGTTGATTCGGCAC CTCCTTCAAAACCAGGCAGAA 61.111 47.619 58.726 58.414 103 241 18 21
157 CGTGTGGAAGGGGAAGCA CACGTGGGATTGGATCATCT 61.111 50 59.57 57.372 148 304 18 20
213 CCCGTTGAGCTGCCGAAC CAAAACCAGGCAGAATAATGTGG 66.667 43.478 61.423 58.503 23 235 18 23
201 TAAGGGGTGCCAATGTCCAT TCCCCAGTACAAGCAAGAGG 50 55 58.993 59.016 173 373 20 20
205 GTGCCAATGTCCATCGCTAG CAGACATCCTTCCCCAGTACA 55 52.381 59.06 58.812 179 383 20 21
199 ATCGCTAGTACAACCAGCCA TGGGCTCAGACATCCTTCC 50 57.895 58.808 58.699 191 389 20 19
192 TCAATGTCTGTGGATGGGGC GCAGAATAATGTGGCCTGGC 55 55 60.034 59.613 35 226 20 20
109 CACCTTCCCACTTCCCTGAG GTCTCCTTCAAAACCAGGCA 60 50 59.674 58.019 135 243 20 20
127 GACAGAATTCTTGCTGGGACC CTCCTTCAAAACCAGGCAGAA 52.381 47.619 58.912 58.414 115 241 21 21
124 GCCAATGTCCATCGCTAGTAC CACGTGGGATTGGATCATCT 52.381 50 58.864 57.372 181 304 21 20
104 GACCACCTTCCCACTTCCC CAAAACCAGGCAGAATAATGTGG 63.158 43.478 59.621 58.503 132 235 19 23
167 GTCTGTGGATGGGGCGAT TGGTTGTACTAGCGATGGACA 61.111 47.619 59.089 58.827 40 206 18 21
117 TGTCCATCGCTAGTACAACCA CGTGGGATTGGATCATCTTGA 47.619 47.619 58.827 57.802 186 302 21 21