Suggested primers
Product size	Left primer	Right primer	Left GC	Right GC	Left TM	Right TM	Left pos	Right pos	Left size	Right size
228	TCAAAGTCTTCCCCTCCACC	CAGAGGCAGAAACAGGAGGA	55	55	58.935	59.02	41	268	20	20
165	TGAGTCACGGGTTCCTCAAA	TTCGTCTGGTAGCTCTGGTG	50	55	58.875	59.111	164	328	20	20
303	AATTCGAGCTCTCCACAGGT	TAGCTCTGGTGGCTCTCTTG	50	55	58.729	58.81	17	319	20	20
288	CTCTCCACAGGTGTCCTCAA	GGTGGCTCTCTTGTTTTCCC	55	55	58.658	58.752	25	312	20	20
307	TCCACAGGTGTCCTCAAAGT	TGGGATTTCGTCTGGTAGCT	50	50	58.493	58.431	28	334	20	20
145	ACATTTCCTCGAGCTCCAGA	ACCACTGAATGTACTGGCTCA	50	47.619	58.438	59.022	98	242	20	21
194	ACCTTCACCTCCATGATGCC	GGTAGCTCTGGTGGCTCTC	55	63.158	59.743	59.18	128	321	20	19
179	TCACCTCCATGATGCCAATG	TGGCTCTCTTGTTTTCCCATG	50	47.619	57.938	58.482	132	310	20	21
197	GTCACGGGTTCCTCAAATGT	TCGATTAAGTCTCCCACCCA	50	50	58.108	57.759	167	363	20	20
123	CCTGCATCACCCCTTTCTTAC	CTCTGGTGGCTCTCTTGTTT	52.381	50	58.631	57.442	194	316	21	20
171	AAGTCTTCCCCTCCACCTCG	GTAAGAAAGGGGTGATGCAGG	60	52.381	60.905	58.631	44	214	20	21