Suggested primers
Product size	Left primer	Right primer	Left GC	Right GC	Left TM	Right TM	Left pos	Right pos	Left size	Right size
173	GTCAGTGCAGGCATGTTTCA	ATAACCGTGAGATTCGCCCT	50	50	59.046	58.879	104	276	20	20
171	GAGCAGGTTTCTTTGGAGCC	AATACTGCCACTCTGACGGT	55	50	59.115	58.733	56	226	20	20
241	CAAAGCGACACACAGAGCAT	AGTGCCTCCCTTCTTTGACT	50	50	59.13	58.56	152	392	20	20
153	GTTTCTTTGGAGCCTCTGGG	CTGACGGTGCTCAAAACAGT	55	50	58.461	58.7	62	214	20	20
144	CATCTCCAAAGCGACACACA	acctgggCTAAAGATAACCGT	50	47.619	58.488	58.806	146	289	20	21
163	CTCCAAAGCGACACACAGAG	tggaacagcaccgatcaaaa	55	45	58.857	58.026	149	311	20	20
117	AGGTCAGTGCAGGCATGTT	CACTCTGACGGTGCTCAAAA	52.632	50	59.542	58.418	102	218	19	20
108	AATGGACAGAGGCGCATCT	GACTCTGGTGGAAAATACTGCC	52.632	50	59.089	58.99	132	239	19	22
102	GCAGGCATGTTTCATTTCTGTG	ACGGTGCTCAAAACAGTCTA	45.455	45	59.009	57.097	110	211	22	20
128	CGCATCTCCAAAGCGACAC	CGTGAGATTCGCCCTGGA	57.895	61.111	59.579	59.107	144	271	19	18
132	AGCGACACACAGAGCATTAA	tgggCTAAAGATAACCGTGAGA	45	45.455	57.544	58.899	155	286	20	22