Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
213 CCTGGGGATTTTGGATGCTG TTGTCGACGTTAGGTTGTGC 55 50 58.883 58.789 129 341 20 20
198 TGGATGCTGATCTGGACTCC CGACGTTAGGTTGTGCTTCC 55 55 58.874 59.212 140 337 20 20
235 TGCCCGCTGACATTATCTCA CTGCTCACCTGATATGCCCT 50 55 59.17 59.236 164 398 20 20
218 TCCTTCCCTCTCATCGTTGG AGCTTCGACTCAGGGAAAGG 55 55 58.805 59.389 66 283 20 20
129 TCGTTGGCCCACTCATAGAT GTCCCATGCAAGTTTGTCTGT 50 47.619 58.504 59.044 79 207 20 21
100 CACAGACAAACTTGCATGGGA GGAGCTTCGACTCAGGGAA 47.619 57.895 59.044 58.722 186 285 21 19
310 TCATCGTTGGCCCACTCATA ATGCCCTGATGAGAGACTGC 50 55 58.8 59.531 76 385 20 20
127 CGTGCCCGCTGACATTATC CAGGGAGCTTCGACTCAGG 57.895 63.158 59.066 59.485 162 288 19 19
262 TGGGGATTTTGGATGCTGATC ACCTGATATGCCCTGATGAGA 47.619 47.619 58.33 58.018 131 392 21 21
221 CTCTCATCGTTGGCCCACT GTAGCCAGGGAGCTTCGAC 57.895 63.158 59.401 59.858 73 293 19 19
150 TGGCCCACTCATAGATACGA CAGCATTGGTGTTTCGGAAA 50 45 57.625 57.209 83 232 20 20
190 CCCGCTGACATTATCTCATCC GGTCCTCCACGAGTTTGTC 52.381 57.895 58.307 57.788 166 355 21 19