Suggested primers
Product size	Left primer	Right primer	Left GC	Right GC	Left TM	Right TM	Left pos	Right pos	Left size	Right size
183	CGAGGGGCAGTAAAAGCATC	GGAGCTGAAACGATGGTGAC	55	55	58.983	58.92	155	337	20	20
145	AAACTGAAGGTAGCCGAGGG	TGGTAAGCAAAGTGGTGTGG	55	50	59.386	58.314	141	285	20	20
219	GGTGACATGCATCTTTGTGTG	TGCACAATCATCTGGCTCAAG	47.619	47.619	58.062	58.911	4	222	21	21
181	GCAGTAAAAGCATCTACTGGGG	CTTTGGAGCTGAAACGATGGT	50	47.619	59.054	58.846	161	341	22	21
133	GGGGCAGTAAAAGCATCTACTG	TGATGTGGTAAGCAAAGTGGTG	50	45.455	59.054	59.116	158	290	22	22
131	CCTGTTGTGAAGATCTTGAGCC	CAAAACAGAAGAGTGAGACGGT	50	45.455	59.254	58.542	188	318	22	22
158	TCGAGCCCATTATGAAACTGA	GGTAAGCAAAGTGGTGTGGTTA	42.857	45.455	57.095	58.784	127	284	21	22
127	CCGAGGGGCAGTAAAAGC	AGCAAAGTGGTGTGGTTAAAGT	61.111	40.909	58.095	58.638	154	280	18	22
119	GTTGTGAAGATCTTGAGCCAGA	AGAGTGAGACGGTAATCCATGA	45.455	45.455	58.332	58.368	191	309	22	22
102	TGCAACCTAAAACTCATCATCGA	GGCTCAAGATCTTCACAACAGG	39.13	50	58.425	59.254	108	209	23	22
186	CTCATCATCGAGCCCATTATGA	TGAGACGGTAATCCATGATGTG	45.455	45.455	57.802	57.941	120	305	22	22
119	GTGAAGATCTTGAGCCAGATGA	AGAAGAGTGAGACGGTAATCCA	45.455	45.455	57.866	58.302	194	312	22	22
205	ACATGCATCTTTGTGTGTTTTGT	TCTGGCTCAAGATCTTCACAAC	34.783	45.455	58.242	58.332	8	212	23	22
183	CGAGGGGCAGTAAAAGCATC	GGAGCTGAAACGATGGTGAC	55	55	58.983	58.92	155	337	20	20
145	AAACTGAAGGTAGCCGAGGG	TGGTAAGCAAAGTGGTGTGG	55	50	59.386	58.314	141	285	20	20
181	GCAGTAAAAGCATCTACTGGGG	CTTTGGAGCTGAAACGATGGT	50	47.619	59.054	58.846	161	341	22	21
219	GGTGACATGCATCTTTGTGTG	TGCACAATCATCTGGCTCAAG	47.619	47.619	58.062	58.911	4	222	21	21
133	GGGGCAGTAAAAGCATCTACTG	TGATGTGGTAAGCAAAGTGGTG	50	45.455	59.054	59.116	158	290	22	22
158	TCGAGCCCATTATGAAACTGA	GGTAAGCAAAGTGGTGTGGTTA	42.857	45.455	57.095	58.784	127	284	21	22
131	CCTGTTGTGAAGATCTTGAGCC	CAAAACAGAAGAGTGAGACGGT	50	45.455	59.254	58.542	188	318	22	22
119	GTTGTGAAGATCTTGAGCCAGA	AGAGTGAGACGGTAATCCATGA	45.455	45.455	58.332	58.368	191	309	22	22
102	TGCAACCTAAAACTCATCATCGA	GGCTCAAGATCTTCACAACAGG	39.13	50	58.425	59.254	108	209	23	22
127	CCGAGGGGCAGTAAAAGC	AGCAAAGTGGTGTGGTTAAAGT	61.111	40.909	58.095	58.638	154	280	18	22
119	GTGAAGATCTTGAGCCAGATGA	AGAAGAGTGAGACGGTAATCCA	45.455	45.455	57.866	58.302	194	312	22	22
186	CTCATCATCGAGCCCATTATGA	TGAGACGGTAATCCATGATGTG	45.455	45.455	57.802	57.941	120	305	22	22
205	ACATGCATCTTTGTGTGTTTTGT	TCTGGCTCAAGATCTTCACAAC	34.783	45.455	58.242	58.332	8	212	23	22
183	CGAGGGGCAGTAAAAGCATC	GGAGCTGAAACGATGGTGAC	55	55	58.983	58.92	155	337	20	20
145	AAACTGAAGGTAGCCGAGGG	TGGTAAGCAAAGTGGTGTGG	55	50	59.386	58.314	141	285	20	20
219	GGTGACATGCATCTTTGTGTG	TGCACAATCATCTGGCTCAAG	47.619	47.619	58.062	58.911	4	222	21	21
131	CCTGTTGTGAAGATCTTGAGCC	CAAAACAGAAGAGTGAGACGGT	50	45.455	59.254	58.542	188	318	22	22
133	GGGGCAGTAAAAGCATCTACTG	TGATGTGGTAAGCAAAGTGGTG	50	45.455	59.054	59.116	158	290	22	22
181	GCAGTAAAAGCATCTACTGGGG	CTTTGGAGCTGAAACGATGGT	50	47.619	59.054	58.846	161	341	22	21
158	TCGAGCCCATTATGAAACTGA	GGTAAGCAAAGTGGTGTGGTTA	42.857	45.455	57.095	58.784	127	284	21	22
127	CCGAGGGGCAGTAAAAGC	AGCAAAGTGGTGTGGTTAAAGT	61.111	40.909	58.095	58.638	154	280	18	22
102	TGCAACCTAAAACTCATCATCGA	GGCTCAAGATCTTCACAACAGG	39.13	50	58.425	59.254	108	209	23	22
119	GTTGTGAAGATCTTGAGCCAGA	AGAGTGAGACGGTAATCCATGA	45.455	45.455	58.332	58.368	191	309	22	22
119	GTGAAGATCTTGAGCCAGATGA	AGAAGAGTGAGACGGTAATCCA	45.455	45.455	57.866	58.302	194	312	22	22
186	CTCATCATCGAGCCCATTATGA	TGAGACGGTAATCCATGATGTG	45.455	45.455	57.802	57.941	120	305	22	22
205	ACATGCATCTTTGTGTGTTTTGT	TCTGGCTCAAGATCTTCACAAC	34.783	45.455	58.242	58.332	8	212	23	22