Suggested primers
Product size	Left primer	Right primer	Left GC	Right GC	Left TM	Right TM	Left pos	Right pos	Left size	Right size
165	CCCAGGTCCGTACATCTGAC	TACCACCACTGCTGCTAACA	60	50	59.54	58.947	94	258	20	20
153	CCGTACATCTGACCTCCTGA	CCACTGCTGCTAACACTTCC	55	55	58.236	58.84	101	253	20	20
245	GTCAACAAACTAAGCGGGGA	ATCAGCACCTACCACCACTG	50	55	58.113	59.385	23	267	20	20
267	TGATGCTGATGAACACGGAG	GTCATCAGCACCTACCACCA	50	55	57.986	59.387	4	270	20	20
251	TCAACAAACTAAGCGGGGAAAA	TAGGGTCATCAGCACCTACC	40.909	55	58.711	58.21	24	274	22	20
169	GCCCCAGGTCCGTACATC	CCTACCACCACTGCTGCTA	66.667	57.895	59.495	58.713	92	260	18	19
151	CGTACATCTGACCTCCTGAAATG	CACTGCTGCTAACACTTCCTTA	47.826	45.455	58.884	58.336	102	252	23	22
137	TGAAGCAAAGTTTCCCAAATGTG	GCACCTACCACCACTGCTG	39.13	63.158	58.559	60.672	127	263	23	19
169	ACCTCCTGAAATGTTTGAAGCA	CCAAAATAGGGTCATCAGCACC	40.909	50	58.435	59.31	112	280	22	22
111	TCTGACCTCCTGAAATGTTTGA	ATGACAACTGTAGCTCTGTAGG	40.909	45.455	57.295	57.272	108	218	22	22
147	TGTGTTGAAGAAGGTGTTACAGA	CCTCAGCAAATTCCAAAATAGGG	39.13	43.478	57.907	57.985	146	292	23	23
101	TGACAATACCTACAGAGCTACAG	CAGCAAATTCCAAAATAGGGTCA	43.478	39.13	57.163	57.596	189	289	23	23
245	GTCAACAAACTAAGCGGGGA	ATCAGCACCTACCACCACTG	50	55	58.113	59.385	23	267	20	20
153	CCGTACATCTGACCTCCTGA	CCACTGCTGCTAACACTTCC	55	55	58.236	58.84	101	253	20	20
267	TGATGCTGATGAACACGGAG	GTCATCAGCACCTACCACCA	50	55	57.986	59.387	4	270	20	20
165	CCCAGGTCCGTACATCTGAC	TACCACCACTGCTGCTAACA	60	50	59.54	58.947	94	258	20	20
251	TCAACAAACTAAGCGGGGAAAA	TAGGGTCATCAGCACCTACC	40.909	55	58.711	58.21	24	274	22	20
169	GCCCCAGGTCCGTACATC	CCTACCACCACTGCTGCTA	66.667	57.895	59.495	58.713	92	260	18	19
151	CGTACATCTGACCTCCTGAAATG	CACTGCTGCTAACACTTCCTTA	47.826	45.455	58.884	58.336	102	252	23	22
169	ACCTCCTGAAATGTTTGAAGCA	CCAAAATAGGGTCATCAGCACC	40.909	50	58.435	59.31	112	280	22	22
137	TGAAGCAAAGTTTCCCAAATGTG	GCACCTACCACCACTGCTG	39.13	63.158	58.559	60.672	127	263	23	19
111	TCTGACCTCCTGAAATGTTTGA	ATGACAACTGTAGCTCTGTAGG	40.909	45.455	57.295	57.272	108	218	22	22
147	TGTGTTGAAGAAGGTGTTACAGA	CCTCAGCAAATTCCAAAATAGGG	39.13	43.478	57.907	57.985	146	292	23	23
101	TGACAATACCTACAGAGCTACAG	CAGCAAATTCCAAAATAGGGTCA	43.478	39.13	57.163	57.596	189	289	23	23
153	CCGTACATCTGACCTCCTGA	CCACTGCTGCTAACACTTCC	55	55	58.236	58.84	101	253	20	20
165	CCCAGGTCCGTACATCTGAC	TACCACCACTGCTGCTAACA	60	50	59.54	58.947	94	258	20	20
245	GTCAACAAACTAAGCGGGGA	ATCAGCACCTACCACCACTG	50	55	58.113	59.385	23	267	20	20
267	TGATGCTGATGAACACGGAG	GTCATCAGCACCTACCACCA	50	55	57.986	59.387	4	270	20	20
251	TCAACAAACTAAGCGGGGAAAA	TAGGGTCATCAGCACCTACC	40.909	55	58.711	58.21	24	274	22	20
169	GCCCCAGGTCCGTACATC	CCTACCACCACTGCTGCTA	66.667	57.895	59.495	58.713	92	260	18	19
169	ACCTCCTGAAATGTTTGAAGCA	CCAAAATAGGGTCATCAGCACC	40.909	50	58.435	59.31	112	280	22	22
151	CGTACATCTGACCTCCTGAAATG	CACTGCTGCTAACACTTCCTTA	47.826	45.455	58.884	58.336	102	252	23	22
137	TGAAGCAAAGTTTCCCAAATGTG	GCACCTACCACCACTGCTG	39.13	63.158	58.559	60.672	127	263	23	19
111	TCTGACCTCCTGAAATGTTTGA	ATGACAACTGTAGCTCTGTAGG	40.909	45.455	57.295	57.272	108	218	22	22
147	TGTGTTGAAGAAGGTGTTACAGA	CCTCAGCAAATTCCAAAATAGGG	39.13	43.478	57.907	57.985	146	292	23	23
101	TGACAATACCTACAGAGCTACAG	CAGCAAATTCCAAAATAGGGTCA	43.478	39.13	57.163	57.596	189	289	23	23