Aliases circ-1216 Scientific name Ovis aries
Organism Sheep Genome Locus chr13:66516141-66524623:+
CircRNA type exon Gene ID n/a
Gene Name RPRD1B Detection pipeline n/a
Sample Type Pitutary gland
Method for Estimation Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer TACCTCTTGGACCCTTACG
Reverse Primer AGTTTCTGGTTCCCTCTGTT
Pubmed 29170496
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
114 ACCCAGAACCAGAAAGACGT AGTCGTCATCCTCCTCCTCT 50 55 58.871 59.077 152 265 20 20
191 TGGAGTACACCCAGAACCAG GCTCTGGGAGGTCAAGACAT 55 55 58.652 59.092 144 334 20 20
254 GCTCGGATGTTGGTGGAGTA TTGTGGCCAGAACCTTCAAC 55 50 59.465 58.601 131 384 20 20
181 CGAATATAACGGGCGCCTG CGTCATCCTCCTCCTCTTGT 57.895 55 58.85 58.52 82 262 19 20
125 CCCAGAACCAGAAAGACGTTTT AGCTTCCAGGATAGTCGTCA 45.455 50 59.05 57.855 153 277 22 20
275 GCATGTCTGTTACTAGCCGA CCTATGCTCTGGGAGGTCAA 50 55 57.421 58.5 65 339 20 20
123 AGTACACCCAGAACCAGAAAGA GGATAGTCGTCATCCTCCTCC 45.455 57.143 58.685 58.836 147 269 22 21
128 GGCTCGGATGTTGGTGGA TCCTCCTCCTCTTGTATTTGCT 61.111 45.455 59.331 58.545 130 257 18 22
151 CAGCTGGCTCGGATGTTG CTTCCAGGATAGTCGTCATCC 61.111 52.381 58.506 57.348 125 275 18 21
172 GAAGACCGACGCCAGCTG GGAGAGTAGCTTCCAGGATAGT 66.667 50 60.811 58.16 113 284 18 22