Aliases circ1566 Scientific name Zea mays
Organism Maize Genome Locus chr7:32640961-32641303:-
CircRNA type n/a Gene ID n/a
Gene Name GRMZM2G109743 Detection pipeline KNIFE
Sample Type third leaves of B73 V3 stage seedlings
Method for Estimation n/a
Circular RNA status Experimented PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer n/a
Reverse Primer n/a
Pubmed 29155438
Aliases circ1566 Scientific name Zea mays
Organism Maize Genome Locus chr7:32640961-32641303:-
CircRNA type n/a Gene ID n/a
Gene Name GRMZM2G109743 Detection pipeline KNIFE
Sample Type third leaves of B73 V3 stage seedlings
Method for Estimation Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer GGAAGGGGTCATCTGTGATTATTAT
Reverse Primer TGAATAAGAAGGATAGAAGCCTTGA
Pubmed 29155438
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
252 TAGATCGAAAGGGCGGGGTT TCTGGGGCTCGGATGTAATT 55 50 60.979 58.493 108 359 20 20
282 CCATAGGATTGTGATAGGACGGA GGGACAGCGGGTTTCTTTC 47.826 57.895 59.168 58.746 43 324 23 19
185 GAAAGGGCGGGGTTGGAC CCGAGGGTCTCAATATGCAAAA 66.667 45.455 60.675 58.721 114 298 18 22
218 TGTTAGATCGAAAGGGCGGG GACAGCGGGTTTCTTTCGAA 55 50 59.823 58.777 105 322 20 20
165 GGTTCGGGTGAGATGGCTT ACCGAGGGTCTCAATATGCA 57.895 50 59.7 58.504 135 299 19 20