Aliases circ1814 Scientific name Zea mays
Organism Maize Genome Locus chr3:218091477-218092961:+
CircRNA type n/a Gene ID n/a
Gene Name GRMZM2G057853 Detection pipeline KNIFE
Sample Type third leaves of B73 V3 stage seedlings
Method for Estimation n/a
Circular RNA status Experimented PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer n/a
Reverse Primer n/a
Aliases circ1814 Scientific name Zea mays
Organism Maize Genome Locus chr3:218091477-218092961:+
CircRNA type n/a Gene ID n/a
Gene Name GRMZM2G057853 Detection pipeline KNIFE
Sample Type third leaves of B73 V3 stage seedlings
Method for Estimation Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer ATTTGGGAGTTTGAAGAAGCTCAT
Reverse Primer CATTTTCAGAATTCCCAGATGTAAT
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
171 GTAGAGCTCCTTCCCGTCTC TGGATCCTGAAAACGACGGA 60 50 58.973 59.029 56 226 20 20
148 TATTGCAGGTACGGGGTCTG CCTCTGCGTCGAAATTTTCCT 55 47.619 59.172 58.923 156 303 20 21
200 TCGGTGGAGATATTGCAGGT CAAAGTCCGACCCTCCCTC 50 63.158 58.504 59.403 146 345 20 19
245 AGATATTGCAGGTACGGGGT CCTCGTTCACACACAAAGTCTA 50 45.455 58.197 58.287 153 397 20 22
222 CTGCCAGTTTCGATTAGGTGT CGTTCACACACAAAGTCTAGTCA 47.619 43.478 58.3 58.89 173 394 21 23
108 TCGATCGAGGGGTAGCTCT CGAAAGTGGATCCTGAAAACGA 57.895 45.455 59.162 58.936 125 232 19 22
243 GTGGAGATATTGCAGGTACGG TCACACACAAAGTCTAGTCATCA 52.381 39.13 58.244 57.47 149 391 21 23