| Aliases | circ1867 | Scientific name | Zea mays | ||||
| Organism | Maize | Genome Locus | chr9:72727417-72728050:+ | ||||
| CircRNA type | n/a | Gene ID | n/a | ||||
| Gene Name | GRMZM2G356423 | Detection pipeline | KNIFE | ||||
| Sample Type | third leaves of B73 V3 stage seedlings | ||||||||
| Method for Estimation | n/a | ||||||||
| Circular RNA status | Experimented | PCR Details | Link to the detailed experimental procedure | ||||||
| Primers (Experimented) | |||||||||
| Primers (Validated) | |||||||||
| Forward Primer | n/a | ||||||||
| Reverse Primer | n/a | ||||||||
| Aliases | circ1867 | Scientific name | Zea mays | ||||
| Organism | Maize | Genome Locus | chr9:72727417-72728050:+ | ||||
| CircRNA type | n/a | Gene ID | n/a | ||||
| Gene Name | GRMZM2G356423 | Detection pipeline | KNIFE | ||||
| Sample Type | third leaves of B73 V3 stage seedlings | ||||||||
| Method for Estimation | Quantitative PCR | ||||||||
| Circular RNA status | Validated | PCR Details | Link to the detailed experimental procedure | ||||||
| Primers (Experimented) | |||||||||
| Primers (Validated) | |||||||||
| Forward Primer | GCCATTTTAGTAGAGCCTGAAATTT | ||||||||
| Reverse Primer | CAAGAGGCTTATCTTGACAGTGTTT | ||||||||
| Suggested primers | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Product size | Left primer | Right primer | Left GC | Right GC | Left TM | Right TM | Left pos | Right pos | Left size | Right size | |
| 325 | GTGGATGGGGTAGATGGGAG | CGTGCGTAGTTAATTGGCCT | 60 | 50 | 58.94 | 58.634 | 40 | 364 | 20 | 20 | |
| 266 | CGAACCATGTATGTGCCAGG | TGCCGTGCGTAGTTAATTGG | 55 | 50 | 58.983 | 58.92 | 102 | 367 | 20 | 20 | |
| 327 | TGGGGTAGATGGGAGGGTAT | GCTTTGCCGTGCGTAGTTAA | 55 | 50 | 58.739 | 59.486 | 45 | 371 | 20 | 20 | |
| 250 | TGTGCCAGGATCAGAACTCA | TGCGTAGTTAATTGGCCTCAC | 50 | 47.619 | 58.649 | 58.648 | 113 | 362 | 20 | 21 | |
| 246 | GCCAGGATCAGAACTCACGT | GCGTAGTTAATTGGCCTCACTTA | 55 | 43.478 | 59.752 | 58.814 | 116 | 361 | 20 | 23 | |