Aliases circ2329 Scientific name Zea mays
Organism Maize Genome Locus chr2:4640128-4640759:+
CircRNA type n/a Gene ID n/a
Gene Name GRMZM2G086489 Detection pipeline KNIFE
Sample Type third leaves of B73 V3 stage seedlings
Method for Estimation n/a
Circular RNA status Experimented PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer n/a
Reverse Primer n/a
Pubmed 29155438
Aliases circ2329 Scientific name Zea mays
Organism Maize Genome Locus chr2:4640128-4640759:+
CircRNA type n/a Gene ID n/a
Gene Name GRMZM2G086489 Detection pipeline KNIFE
Sample Type third leaves of B73 V3 stage seedlings
Method for Estimation Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer TCAATGGATAACTGCTAGTCAGAGG
Reverse Primer AGCATATCGTAGAAGCGCAGTC
Pubmed 29155438
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
292 CCCGGCGTGTTCTATTCATG CGCTAGGTAGGTAGGTGGTG 55 60 59.063 58.97 28 319 20 20
187 GCCACCTCTGTACTGTACGT GGTAGGTAGGTGGTGGATGG 55 60 59.113 58.871 128 314 20 20
183 CGTGCCACCTCTGTACTGTA AGGTGGTGGATGGATGGATG 55 55 59.114 59.151 125 307 20 20
219 TTGAAGACGCTGACACTGTG GGTCGCTAGGTAGGTAGGTG 50 60 58.429 58.683 104 322 20 20
137 GTACTTCACGCACGGCATC AGGTAGGTGGTGGATGGATG 57.895 55 59.292 58.488 175 311 19 20
237 CCGGCGAAGAATTGAAGACG ATGCATGGGTCGCTAGGTAG 55 55 59.63 59.318 93 329 20 20
197 CTGGCCGGCGAAGAATTG TGGATGGAAGGACAAGGACA 61.111 50 59.206 58.259 89 285 18 20
174 GGCCGGCGAAGAATTGAAG GAGGACATGGACAGGATGCA 57.895 55 59.571 59.457 91 264 19 20
164 CGAAGAATTGAAGACGCTGACA ACATGGACAGGATGCACTTG 45.455 50 59.269 58.17 97 260 22 20
203 GCGTGCCACCTCTGTACT CATGGGTCGCTAGGTAGGTA 61.111 55 59.343 58.009 124 326 18 20
105 GAGTACTTCACGCACGGC AGGACAAGGACATGAGGACA 61.111 50 58.54 57.97 173 277 18 20
265 CGTGTTCTATTCATGCGACAAC TGGATGGATGGATGGATGGA 45.455 50 58.34 57.863 33 297 22 20
133 GCCGGAGTACTTCACGCA TGGATGGATGGATGGATGGA 61.111 50 59.74 57.863 169 301 18 20
130 CTGGCGCCGGAGTACTTC TGGATGGATGGATGGAAGGA 66.667 50 60.203 57.789 164 293 18 20