| Aliases | hsa_circ_0072765 | Scientific name | Homo sapiens (hg19) | ||||
| Organism | Human | Genome Locus | chr5:68471223-68471364:+ | ||||
| CircRNA type | . | Gene ID | ENSG00000134057.10 | ||||
| Gene Name | CCNB1 | Detection pipeline | . | ||||
| Sample Type | K562; Hepg2; H1hesc; Gm12878; A549; Sknshra | ||||||||
| Method for Estimation | RNA-Seq with Rnase R resistance | ||||||||
| Circular RNA status | Experimented | PCR Details | Link to the detailed experimental procedure | ||||||
| Primers (Experimented) | |||||||||
| Primers (Validated) | |||||||||
| Forward Primer | . | ||||||||
| Reverse Primer | . | ||||||||
| Aliases | hsa_circ_0072765 | Scientific name | Homo sapiens (hg19) | ||||
| Organism | Human | Genome Locus | chr5:68471223-68471364:+ | ||||
| CircRNA type | N/A | Gene ID | ENSG00000134057.10 | ||||
| Gene Name | CCNB1 | Detection pipeline | N/A | ||||
| Sample Type | Irradiated Hepatic Stellate Cell (HSC) | ||||||||
| Method for Estimation | Microarray and Quantitative PCR | ||||||||
| Circular RNA status | Validated | PCR Details | Link to the detailed experimental procedure | ||||||
| Primers (Experimented) | |||||||||
| Primers (Validated) | |||||||||
| Forward Primer | TTGCAGCAGGAGCTTTTTGC | ||||||||
| Reverse Primer | GGCCAAAGTATGTTGCTCGAC | ||||||||
| Aliases | hsa_circ_0072765 | Scientific name | Homo sapiens (hg19) | ||||
| Organism | Human | Genome Locus | chr5:68471223-68471364:+ | ||||
| CircRNA type | N/A | Gene ID | ENSG00000134057.10 | ||||
| Gene Name | CCNB1 | Detection pipeline | N/A | ||||
| Sample Type | Irradiated Hepatic Stellate Cell (HSC) | ||||||||
| Method for Estimation | Microarray and Quantitative PCR | ||||||||
| Circular RNA status | Validated | PCR Details | Link to the detailed experimental procedure | ||||||
| Primers (Experimented) | |||||||||
| Primers (Validated) | |||||||||
| Forward Primer | TTGCAGCAGGAGCTTTTTGC | ||||||||
| Reverse Primer | GGCCAAAGTATGTTGCTCGAC | ||||||||
| Suggested primers | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Product size | Left primer | Right primer | Left GC | Right GC | Left TM | Right TM | Left pos | Right pos | Left size | Right size | |
| 165 | TGGTGAATGGGTTGATGTCG | GGGACACAGCTTACCCATTC | 50 | 55 | 58.18 | 58.249 | 190 | 354 | 20 | 20 | |
| 169 | ACATGGGTTTTGTTTCAGGTTG | CGACATCAACCCATTCACCA | 40.909 | 50 | 57.864 | 58.18 | 41 | 209 | 22 | 20 | |
| 309 | TGGGTTTTGTTTCAGGTTGATG | GACACAGCTTACCCATTCACC | 40.909 | 52.381 | 57.601 | 58.915 | 44 | 352 | 22 | 21 | |
| 167 | GGTTTTGTTTCAGGTTGATGTCG | GCTCGACATCAACCCATTCA | 43.478 | 50 | 58.958 | 58.262 | 46 | 212 | 23 | 20 | |
| 371 | AGGTTAGTGCCAAAGGAAAATTT | AGCTTAGGAGTTCTGTGGGAC | 34.783 | 52.381 | 57.113 | 59.097 | 0 | 370 | 23 | 21 | |
| 167 | GGTTTTGTTTCAGGTTGATGTCG | GCTCGACATCAACCCATTCA | 43.478 | 50 | 58.958 | 58.262 | 46 | 212 | 23 | 20 | |
| 309 | TGGGTTTTGTTTCAGGTTGATG | GACACAGCTTACCCATTCACC | 40.909 | 52.381 | 57.601 | 58.915 | 44 | 352 | 22 | 21 | |
| 169 | ACATGGGTTTTGTTTCAGGTTG | CGACATCAACCCATTCACCA | 40.909 | 50 | 57.864 | 58.18 | 41 | 209 | 22 | 20 | |
| 165 | TGGTGAATGGGTTGATGTCG | GGGACACAGCTTACCCATTC | 50 | 55 | 58.18 | 58.249 | 190 | 354 | 20 | 20 | |
| 371 | AGGTTAGTGCCAAAGGAAAATTT | AGCTTAGGAGTTCTGTGGGAC | 34.783 | 52.381 | 57.113 | 59.097 | 0 | 370 | 23 | 21 | |