Suggested primers
Product size	Left primer	Right primer	Left GC	Right GC	Left TM	Right TM	Left pos	Right pos	Left size	Right size
118	GTCAGTGCAGGCATGTTTCA	CCTGGAGATGCACAGTCTCA	50	55	59.046	59.1	104	221	20	20
273	TCTTCGGGAGCAGGTTTCTT	TAGGCGGGTCTCCAGAAATC	50	55	58.943	58.879	49	321	20	20
167	CAAAGCGACACACAGAGCAT	GCGGGTCTCCAGAAATCAAG	50	55	59.13	58.626	152	318	20	20
105	CTCCAAAGCGACACACAGAG	TTCCGGATGTCATTCGAGGA	55	50	58.857	58.519	149	253	20	20
179	CATCTCCAAAGCGACACACA	AAATAGGCGGGTCTCCAGAA	50	50	58.488	58.42	146	324	20	20
246	AATGGACAGAGGCGCATCT	GCTAGACCAATCACGGATCG	52.632	55	59.089	58.228	132	377	19	20
110	GCAGGCATGTTTCATTTCTGTG	TGGAGATGCACAGTCTCAGTA	45.455	47.619	59.009	58.192	110	219	22	21
112	AGGTCAGTGCAGGCATGTT	TGCACAGTCTCAGTACAGTCT	52.632	47.619	59.542	58.412	102	213	19	21
124	AGCGACACACAGAGCATTAA	GGTGGAGATTCAAGAAAGCAGT	45	45.455	57.544	58.584	155	278	20	22
173	CGCATCTCCAAAGCGACAC	GGGTCTCCAGAAATCAAGGTT	57.895	47.619	59.579	57.558	144	316	19	21
189	AGACTGTACTGAGACTGTGCA	AAAAGCTAGACCAATCACGGA	47.619	42.857	58.412	57.304	193	381	21	21