Suggested primers |
Product size |
Left primer |
Right primer |
Left GC |
Right GC |
Left TM |
Right TM |
Left pos |
Right pos |
Left size |
Right size |
213 |
CCTGGGGATTTTGGATGCTG |
TTGTCGACGTTAGGTTGTGC |
55 |
50 |
58.883 |
58.789 |
129 |
341 |
20 |
20 |
198 |
TGGATGCTGATCTGGACTCC |
CGACGTTAGGTTGTGCTTCC |
55 |
55 |
58.874 |
59.212 |
140 |
337 |
20 |
20 |
235 |
TGCCCGCTGACATTATCTCA |
CTGCTCACCTGATATGCCCT |
50 |
55 |
59.17 |
59.236 |
164 |
398 |
20 |
20 |
218 |
TCCTTCCCTCTCATCGTTGG |
AGCTTCGACTCAGGGAAAGG |
55 |
55 |
58.805 |
59.389 |
66 |
283 |
20 |
20 |
129 |
TCGTTGGCCCACTCATAGAT |
GTCCCATGCAAGTTTGTCTGT |
50 |
47.619 |
58.504 |
59.044 |
79 |
207 |
20 |
21 |
100 |
CACAGACAAACTTGCATGGGA |
GGAGCTTCGACTCAGGGAA |
47.619 |
57.895 |
59.044 |
58.722 |
186 |
285 |
21 |
19 |
310 |
TCATCGTTGGCCCACTCATA |
ATGCCCTGATGAGAGACTGC |
50 |
55 |
58.8 |
59.531 |
76 |
385 |
20 |
20 |
127 |
CGTGCCCGCTGACATTATC |
CAGGGAGCTTCGACTCAGG |
57.895 |
63.158 |
59.066 |
59.485 |
162 |
288 |
19 |
19 |
262 |
TGGGGATTTTGGATGCTGATC |
ACCTGATATGCCCTGATGAGA |
47.619 |
47.619 |
58.33 |
58.018 |
131 |
392 |
21 |
21 |
221 |
CTCTCATCGTTGGCCCACT |
GTAGCCAGGGAGCTTCGAC |
57.895 |
63.158 |
59.401 |
59.858 |
73 |
293 |
19 |
19 |
150 |
TGGCCCACTCATAGATACGA |
CAGCATTGGTGTTTCGGAAA |
50 |
45 |
57.625 |
57.209 |
83 |
232 |
20 |
20 |
190 |
CCCGCTGACATTATCTCATCC |
GGTCCTCCACGAGTTTGTC |
52.381 |
57.895 |
58.307 |
57.788 |
166 |
355 |
21 |
19 |