Suggested primers
Product size	Left primer	Right primer	Left GC	Right GC	Left TM	Right TM	Left pos	Right pos	Left size	Right size
100	GCCAGATTATGAACGGAGGC	TTCCAGGGAAACAAAGTGACA	55	42.857	58.77	57.626	161	260	20	21
104	AAGATTGCTCCAGATCTGCC	AAGTGACATGCTTCCATAGGG	50	47.619	57.655	57.712	144	247	20	21
110	ATTGCTCCAGATCTGCCAGA	AGGGAAACAAAGTGACATGCT	50	42.857	58.789	58.051	147	256	20	21
157	TTATGAACGGAGGCCCTTGG	AGCTTGTAAGTTTAGAACAGCCT	55	39.13	59.745	58.026	167	323	20	23
158	AGGCCCTTGGGATTTGGATC	AGGAATGTCAGAGCTTGTAAGT	55	40.909	59.737	57.101	177	334	20	22
173	AGTAATTTGGCTGCATTTGTCA	AGGGATTTCTGAGAGGCGAT	36.364	50	57.13	58.2	58	230	22	20
118	AGCTTGTAAGATTGCTCCAGA	GGAAACAAAGTGACATGCTTCC	42.857	45.455	57.011	58.357	137	254	21	22
178	CAAAAGAACACCTGTATTTCGGA	GTGACAGGCAGGATCCAAATC	39.13	52.381	57.317	58.98	30	207	23	21