Suggested primers |
Product size |
Left primer |
Right primer |
Left GC |
Right GC |
Left TM |
Right TM |
Left pos |
Right pos |
Left size |
Right size |
250 |
aaaattagccgggtgtggtg |
ACACAACTGCTTGGCTCTAC |
50 |
50 |
58.747 |
58.116 |
53 |
302 |
20 |
20 |
114 |
cggaaggttgaggcagaaga |
GTGAGGCCATACaatttcgct |
55 |
47.619 |
59.678 |
58.983 |
98 |
211 |
20 |
21 |
133 |
ccagctactcggaaggttga |
ACCAAGACTTGTGAGGCCAT |
55 |
50 |
59.104 |
59.226 |
89 |
221 |
20 |
20 |
106 |
ggttgaggcagaagaatcgt |
AGGCCATACaatttcgctctt |
50 |
42.857 |
57.899 |
57.995 |
103 |
208 |
20 |
21 |
118 |
agcgaaattGTATGGCCTCAC |
TGGAATACACAACTGCTTGGC |
47.619 |
47.619 |
58.983 |
59.114 |
191 |
308 |
21 |
21 |
123 |
cctggatgacaagagcgaaa |
ACAACTGCTTGGCTCTACTTTG |
50 |
45.455 |
57.901 |
59.119 |
178 |
300 |
20 |
22 |
130 |
ctcggaaggttgaggcaga |
GTAGACCAAGACTTGTGAGGC |
57.895 |
52.381 |
59.025 |
58.31 |
96 |
225 |
19 |
21 |
212 |
aggtggaggttgcagtgag |
TCTACCATTCACATAGGTCCGT |
57.895 |
45.455 |
59.243 |
58.361 |
134 |
345 |
19 |
22 |
105 |
agaatcgtttgaacctgggag |
CAAGACTTGTGAGGCCATACa |
47.619 |
47.619 |
57.941 |
57.942 |
115 |
219 |
21 |
21 |
123 |
agagcgaaattGTATGGCCT |
TCCTGGAATACACAACTGCTT |
45 |
42.857 |
57.282 |
57.212 |
189 |
311 |
20 |
21 |
179 |
ccgagatcacgccattgc |
AGGTCCGTGGATAGTTTCTTTC |
61.111 |
45.455 |
58.983 |
57.796 |
153 |
331 |
18 |
22 |
219 |
cagcctggatgacaagagc |
TGTCTGAAAAGCACTACCCTT |
57.895 |
42.857 |
58.222 |
57.143 |
175 |
393 |
19 |
21 |
208 |
cctgggaggtggaggttg |
ACATAGGTCCGTGGATAGTTTCT |
66.667 |
43.478 |
58.918 |
58.712 |
128 |
335 |
18 |
23 |
109 |
ggcagaagaatcgtttgaacct |
AGACTTGTGAGGCCATACaatt |
45.455 |
40.909 |
59.186 |
57.693 |
109 |
217 |
22 |
22 |
144 |
ggaggtggaggttgcagt |
TTCTTCACACTACAAAAGGCAC |
61.111 |
40.909 |
58.838 |
57.107 |
132 |
275 |
18 |
22 |
222 |
CGGCCCATTCTTCAACAAGT |
ACCAAGACTTGTGAGGCCAT |
50 |
50 |
58.752 |
59.226 |
0 |
221 |
20 |
20 |
244 |
TCATGCTGATCTCAAGCCAGA |
ACACAACTGCTTGGCTCTAC |
47.619 |
50 |
59.164 |
58.116 |
59 |
302 |
21 |
20 |
204 |
CGGCCAGTCATGTATCAAAGA |
TCTACCATTCACATAGGTCCGT |
47.619 |
45.455 |
58.095 |
58.361 |
142 |
345 |
21 |
22 |
169 |
GTCGGCCAGTCATGTATCAA |
TGGAATACACAACTGCTTGGC |
50 |
47.619 |
57.69 |
59.114 |
140 |
308 |
20 |
21 |
153 |
TCTACAATCTCGGTACTACAGGT |
AGGTCCGTGGATAGTTTCTTTC |
43.478 |
45.455 |
57.892 |
57.796 |
179 |
331 |
23 |
22 |
134 |
GGTGGATCCTGTTCGGCA |
GTAGACCAAGACTTGTGAGGC |
61.111 |
52.381 |
59.331 |
58.31 |
92 |
225 |
18 |
21 |
211 |
TGTTCGGCAGCCTTACAG |
TCCTGGAATACACAACTGCTT |
55.556 |
42.857 |
57.306 |
57.212 |
101 |
311 |
18 |
21 |
262 |
GCCAGAGAATATTATGTTGGTGG |
ACATAGGTCCGTGGATAGTTTCT |
43.478 |
43.478 |
57.304 |
58.712 |
74 |
335 |
23 |
23 |
222 |
CGGCCCATTCTTCAACAAGT |
ACCAAGACTTGTGAGGCCAT |
50 |
50 |
58.752 |
59.226 |
0 |
221 |
20 |
20 |
244 |
TCATGCTGATCTCAAGCCAGA |
ACACAACTGCTTGGCTCTAC |
47.619 |
50 |
59.164 |
58.116 |
59 |
302 |
21 |
20 |
169 |
GTCGGCCAGTCATGTATCAA |
TGGAATACACAACTGCTTGGC |
50 |
47.619 |
57.69 |
59.114 |
140 |
308 |
20 |
21 |
134 |
GGTGGATCCTGTTCGGCA |
GTAGACCAAGACTTGTGAGGC |
61.111 |
52.381 |
59.331 |
58.31 |
92 |
225 |
18 |
21 |
204 |
CGGCCAGTCATGTATCAAAGA |
TCTACCATTCACATAGGTCCGT |
47.619 |
45.455 |
58.095 |
58.361 |
142 |
345 |
21 |
22 |
211 |
TGTTCGGCAGCCTTACAG |
TCCTGGAATACACAACTGCTT |
55.556 |
42.857 |
57.306 |
57.212 |
101 |
311 |
18 |
21 |
153 |
TCTACAATCTCGGTACTACAGGT |
AGGTCCGTGGATAGTTTCTTTC |
43.478 |
45.455 |
57.892 |
57.796 |
179 |
331 |
23 |
22 |
262 |
GCCAGAGAATATTATGTTGGTGG |
ACATAGGTCCGTGGATAGTTTCT |
43.478 |
43.478 |
57.304 |
58.712 |
74 |
335 |
23 |
23 |
222 |
CGGCCCATTCTTCAACAAGT |
ACCAAGACTTGTGAGGCCAT |
50 |
50 |
58.752 |
59.226 |
0 |
221 |
20 |
20 |
244 |
TCATGCTGATCTCAAGCCAGA |
ACACAACTGCTTGGCTCTAC |
47.619 |
50 |
59.164 |
58.116 |
59 |
302 |
21 |
20 |
169 |
GTCGGCCAGTCATGTATCAA |
TGGAATACACAACTGCTTGGC |
50 |
47.619 |
57.69 |
59.114 |
140 |
308 |
20 |
21 |
134 |
GGTGGATCCTGTTCGGCA |
GTAGACCAAGACTTGTGAGGC |
61.111 |
52.381 |
59.331 |
58.31 |
92 |
225 |
18 |
21 |
204 |
CGGCCAGTCATGTATCAAAGA |
TCTACCATTCACATAGGTCCGT |
47.619 |
45.455 |
58.095 |
58.361 |
142 |
345 |
21 |
22 |
211 |
TGTTCGGCAGCCTTACAG |
TCCTGGAATACACAACTGCTT |
55.556 |
42.857 |
57.306 |
57.212 |
101 |
311 |
18 |
21 |
153 |
TCTACAATCTCGGTACTACAGGT |
AGGTCCGTGGATAGTTTCTTTC |
43.478 |
45.455 |
57.892 |
57.796 |
179 |
331 |
23 |
22 |
262 |
GCCAGAGAATATTATGTTGGTGG |
ACATAGGTCCGTGGATAGTTTCT |
43.478 |
43.478 |
57.304 |
58.712 |
74 |
335 |
23 |
23 |