Suggested primers
Product size	Left primer	Right primer	Left GC	Right GC	Left TM	Right TM	Left pos	Right pos	Left size	Right size
243	TTTTGCAAGAAAAGGGGCCT	AAGACAGATGAAGGGAGGGG	45	55	58.494	58.411	138	380	20	20
114	AAGGGGCCTTCTGTGGATTG	GCTCTTGCCGAATGACTTCT	55	50	59.96	58.268	149	262	20	20
164	GCCTTCTGTGGATTGGGGAA	TGTGATGATGCTGTGGTGAG	55	50	59.96	57.896	154	317	20	20
182	ACTTCTATTTTCACAGCACACCA	TGCCGAATGACTTCTTGGAAC	39.13	47.619	58.54	58.849	76	257	23	21
136	CTGGATGGATTTCGGAAGCT	TGGAACTGAGAAGCACCATCT	50	47.619	57.661	59.019	107	242	20	21
182	TCTATTTTCACAGCACACCAAAA	TCTTGCCGAATGACTTCTTGG	34.783	47.619	57.156	58.573	79	260	23	21
124	CTGGATGGATTTCGGAAGCTATT	GCACCATCTTGAGACATTGCT	43.478	47.619	58.607	58.91	107	230	23	21
187	TGCTGGTTGTAACAATGACTTCT	TCTTGGAACTGAGAAGCACCA	39.13	47.619	58.54	59.232	59	245	23	21
114	AAGGGGCCTTCTGTGGATTG	GCTCTTGCCGAATGACTTCT	55	50	59.96	58.268	149	262	20	20
164	GCCTTCTGTGGATTGGGGAA	TGTGATGATGCTGTGGTGAG	55	50	59.96	57.896	154	317	20	20
243	TTTTGCAAGAAAAGGGGCCT	AAGACAGATGAAGGGAGGGG	45	55	58.494	58.411	138	380	20	20
136	CTGGATGGATTTCGGAAGCT	TGGAACTGAGAAGCACCATCT	50	47.619	57.661	59.019	107	242	20	21
124	CTGGATGGATTTCGGAAGCTATT	GCACCATCTTGAGACATTGCT	43.478	47.619	58.607	58.91	107	230	23	21
187	TGCTGGTTGTAACAATGACTTCT	TCTTGGAACTGAGAAGCACCA	39.13	47.619	58.54	59.232	59	245	23	21
182	ACTTCTATTTTCACAGCACACCA	TGCCGAATGACTTCTTGGAAC	39.13	47.619	58.54	58.849	76	257	23	21
182	TCTATTTTCACAGCACACCAAAA	TCTTGCCGAATGACTTCTTGG	34.783	47.619	57.156	58.573	79	260	23	21
114	AAGGGGCCTTCTGTGGATTG	GCTCTTGCCGAATGACTTCT	55	50	59.96	58.268	149	262	20	20
164	GCCTTCTGTGGATTGGGGAA	TGTGATGATGCTGTGGTGAG	55	50	59.96	57.896	154	317	20	20
243	TTTTGCAAGAAAAGGGGCCT	AAGACAGATGAAGGGAGGGG	45	55	58.494	58.411	138	380	20	20
136	CTGGATGGATTTCGGAAGCT	TGGAACTGAGAAGCACCATCT	50	47.619	57.661	59.019	107	242	20	21
124	CTGGATGGATTTCGGAAGCTATT	GCACCATCTTGAGACATTGCT	43.478	47.619	58.607	58.91	107	230	23	21
182	ACTTCTATTTTCACAGCACACCA	TGCCGAATGACTTCTTGGAAC	39.13	47.619	58.54	58.849	76	257	23	21
187	TGCTGGTTGTAACAATGACTTCT	TCTTGGAACTGAGAAGCACCA	39.13	47.619	58.54	59.232	59	245	23	21
182	TCTATTTTCACAGCACACCAAAA	TCTTGCCGAATGACTTCTTGG	34.783	47.619	57.156	58.573	79	260	23	21