Suggested primers |
Product size |
Left primer |
Right primer |
Left GC |
Right GC |
Left TM |
Right TM |
Left pos |
Right pos |
Left size |
Right size |
165 |
CCCAGGTCCGTACATCTGAC |
TACCACCACTGCTGCTAACA |
60 |
50 |
59.54 |
58.947 |
94 |
258 |
20 |
20 |
153 |
CCGTACATCTGACCTCCTGA |
CCACTGCTGCTAACACTTCC |
55 |
55 |
58.236 |
58.84 |
101 |
253 |
20 |
20 |
245 |
GTCAACAAACTAAGCGGGGA |
ATCAGCACCTACCACCACTG |
50 |
55 |
58.113 |
59.385 |
23 |
267 |
20 |
20 |
267 |
TGATGCTGATGAACACGGAG |
GTCATCAGCACCTACCACCA |
50 |
55 |
57.986 |
59.387 |
4 |
270 |
20 |
20 |
251 |
TCAACAAACTAAGCGGGGAAAA |
TAGGGTCATCAGCACCTACC |
40.909 |
55 |
58.711 |
58.21 |
24 |
274 |
22 |
20 |
169 |
GCCCCAGGTCCGTACATC |
CCTACCACCACTGCTGCTA |
66.667 |
57.895 |
59.495 |
58.713 |
92 |
260 |
18 |
19 |
151 |
CGTACATCTGACCTCCTGAAATG |
CACTGCTGCTAACACTTCCTTA |
47.826 |
45.455 |
58.884 |
58.336 |
102 |
252 |
23 |
22 |
137 |
TGAAGCAAAGTTTCCCAAATGTG |
GCACCTACCACCACTGCTG |
39.13 |
63.158 |
58.559 |
60.672 |
127 |
263 |
23 |
19 |
169 |
ACCTCCTGAAATGTTTGAAGCA |
CCAAAATAGGGTCATCAGCACC |
40.909 |
50 |
58.435 |
59.31 |
112 |
280 |
22 |
22 |
111 |
TCTGACCTCCTGAAATGTTTGA |
ATGACAACTGTAGCTCTGTAGG |
40.909 |
45.455 |
57.295 |
57.272 |
108 |
218 |
22 |
22 |
147 |
TGTGTTGAAGAAGGTGTTACAGA |
CCTCAGCAAATTCCAAAATAGGG |
39.13 |
43.478 |
57.907 |
57.985 |
146 |
292 |
23 |
23 |
101 |
TGACAATACCTACAGAGCTACAG |
CAGCAAATTCCAAAATAGGGTCA |
43.478 |
39.13 |
57.163 |
57.596 |
189 |
289 |
23 |
23 |
245 |
GTCAACAAACTAAGCGGGGA |
ATCAGCACCTACCACCACTG |
50 |
55 |
58.113 |
59.385 |
23 |
267 |
20 |
20 |
153 |
CCGTACATCTGACCTCCTGA |
CCACTGCTGCTAACACTTCC |
55 |
55 |
58.236 |
58.84 |
101 |
253 |
20 |
20 |
267 |
TGATGCTGATGAACACGGAG |
GTCATCAGCACCTACCACCA |
50 |
55 |
57.986 |
59.387 |
4 |
270 |
20 |
20 |
165 |
CCCAGGTCCGTACATCTGAC |
TACCACCACTGCTGCTAACA |
60 |
50 |
59.54 |
58.947 |
94 |
258 |
20 |
20 |
251 |
TCAACAAACTAAGCGGGGAAAA |
TAGGGTCATCAGCACCTACC |
40.909 |
55 |
58.711 |
58.21 |
24 |
274 |
22 |
20 |
169 |
GCCCCAGGTCCGTACATC |
CCTACCACCACTGCTGCTA |
66.667 |
57.895 |
59.495 |
58.713 |
92 |
260 |
18 |
19 |
151 |
CGTACATCTGACCTCCTGAAATG |
CACTGCTGCTAACACTTCCTTA |
47.826 |
45.455 |
58.884 |
58.336 |
102 |
252 |
23 |
22 |
169 |
ACCTCCTGAAATGTTTGAAGCA |
CCAAAATAGGGTCATCAGCACC |
40.909 |
50 |
58.435 |
59.31 |
112 |
280 |
22 |
22 |
137 |
TGAAGCAAAGTTTCCCAAATGTG |
GCACCTACCACCACTGCTG |
39.13 |
63.158 |
58.559 |
60.672 |
127 |
263 |
23 |
19 |
111 |
TCTGACCTCCTGAAATGTTTGA |
ATGACAACTGTAGCTCTGTAGG |
40.909 |
45.455 |
57.295 |
57.272 |
108 |
218 |
22 |
22 |
147 |
TGTGTTGAAGAAGGTGTTACAGA |
CCTCAGCAAATTCCAAAATAGGG |
39.13 |
43.478 |
57.907 |
57.985 |
146 |
292 |
23 |
23 |
101 |
TGACAATACCTACAGAGCTACAG |
CAGCAAATTCCAAAATAGGGTCA |
43.478 |
39.13 |
57.163 |
57.596 |
189 |
289 |
23 |
23 |
153 |
CCGTACATCTGACCTCCTGA |
CCACTGCTGCTAACACTTCC |
55 |
55 |
58.236 |
58.84 |
101 |
253 |
20 |
20 |
165 |
CCCAGGTCCGTACATCTGAC |
TACCACCACTGCTGCTAACA |
60 |
50 |
59.54 |
58.947 |
94 |
258 |
20 |
20 |
245 |
GTCAACAAACTAAGCGGGGA |
ATCAGCACCTACCACCACTG |
50 |
55 |
58.113 |
59.385 |
23 |
267 |
20 |
20 |
267 |
TGATGCTGATGAACACGGAG |
GTCATCAGCACCTACCACCA |
50 |
55 |
57.986 |
59.387 |
4 |
270 |
20 |
20 |
251 |
TCAACAAACTAAGCGGGGAAAA |
TAGGGTCATCAGCACCTACC |
40.909 |
55 |
58.711 |
58.21 |
24 |
274 |
22 |
20 |
169 |
GCCCCAGGTCCGTACATC |
CCTACCACCACTGCTGCTA |
66.667 |
57.895 |
59.495 |
58.713 |
92 |
260 |
18 |
19 |
169 |
ACCTCCTGAAATGTTTGAAGCA |
CCAAAATAGGGTCATCAGCACC |
40.909 |
50 |
58.435 |
59.31 |
112 |
280 |
22 |
22 |
151 |
CGTACATCTGACCTCCTGAAATG |
CACTGCTGCTAACACTTCCTTA |
47.826 |
45.455 |
58.884 |
58.336 |
102 |
252 |
23 |
22 |
137 |
TGAAGCAAAGTTTCCCAAATGTG |
GCACCTACCACCACTGCTG |
39.13 |
63.158 |
58.559 |
60.672 |
127 |
263 |
23 |
19 |
111 |
TCTGACCTCCTGAAATGTTTGA |
ATGACAACTGTAGCTCTGTAGG |
40.909 |
45.455 |
57.295 |
57.272 |
108 |
218 |
22 |
22 |
147 |
TGTGTTGAAGAAGGTGTTACAGA |
CCTCAGCAAATTCCAAAATAGGG |
39.13 |
43.478 |
57.907 |
57.985 |
146 |
292 |
23 |
23 |
101 |
TGACAATACCTACAGAGCTACAG |
CAGCAAATTCCAAAATAGGGTCA |
43.478 |
39.13 |
57.163 |
57.596 |
189 |
289 |
23 |
23 |