| Aliases | circ-8710 | Scientific name | Ovis aries | ||||
| Organism | Sheep | Genome Locus | chr7:32399792-32444068:- | ||||
| CircRNA type | intron | Gene ID | n/a | ||||
| Gene Name | ENSOARG00000020072 | Detection pipeline | n/a | ||||
| Sample Type | Pitutary gland | ||||||||
| Method for Estimation | Quantitative PCR | ||||||||
| Circular RNA status | Validated | PCR Details | Link to the detailed experimental procedure | ||||||
| Primers (Experimented) | |||||||||
| Primers (Validated) | |||||||||
| Forward Primer | GACACAGAGAACAGGAGGAGC | ||||||||
| Reverse Primer | TCCATGTGTAGCGAAACGAA | ||||||||
| Suggested primers | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Product size | Left primer | Right primer | Left GC | Right GC | Left TM | Right TM | Left pos | Right pos | Left size | Right size | |
| 175 | TCTGCTGATCCCGTTGTTCT | TGCTGACATGTGAGAAGCAAC | 50 | 47.619 | 59.023 | 59.124 | 50 | 224 | 20 | 21 | |
| 307 | CGATTCTGCTGATCCCGTTG | CGGAAGATGAAGAGGCTTGAC | 55 | 52.381 | 59.067 | 58.725 | 46 | 352 | 20 | 21 | |
| 232 | CAACCGATTCTGCTGATCCC | TGAGAATAAAGCTGTGGGAGGA | 55 | 45.455 | 58.697 | 58.819 | 42 | 273 | 20 | 22 | |
| 312 | TCTCTCAACCGATTCTGCTGA | AGATGAAGAGGCTTGACAAGATA | 47.619 | 39.13 | 58.829 | 57.053 | 37 | 348 | 21 | 23 | |
| 203 | CAGGCTGAAACTGTGCCATC | CAACTTCAGGATGCTATTCGGA | 55 | 45.455 | 59.475 | 58.203 | 168 | 370 | 20 | 22 | |
| 290 | TGCTGATCCCGTTGTTCTTTG | GAGGCTTGACAAGATATTGGTGA | 47.619 | 43.478 | 59.121 | 58.483 | 52 | 341 | 21 | 23 | |