| Aliases | circ-4460 | Scientific name | Ovis aries | ||||
| Organism | Sheep | Genome Locus | chr22:23629427-23633603:+ | ||||
| CircRNA type | exon | Gene ID | n/a | ||||
| Gene Name | ENSOARG00000005177 | Detection pipeline | n/a | ||||
| Sample Type | Pitutary gland | ||||||||
| Method for Estimation | Quantitative PCR | ||||||||
| Circular RNA status | Validated | PCR Details | Link to the detailed experimental procedure | ||||||
| Primers (Experimented) | |||||||||
| Primers (Validated) | |||||||||
| Forward Primer | CAAAACACCAAAGAACAACG | ||||||||
| Reverse Primer | TCCGAATAGCATCCTGAAGT | ||||||||
| Suggested primers | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Product size | Left primer | Right primer | Left GC | Right GC | Left TM | Right TM | Left pos | Right pos | Left size | Right size | |
| 106 | ACAGAGATCTAAAGGCTGGCA | TGTCAATCACCTTTGCAGCA | 47.619 | 45 | 58.809 | 58.31 | 143 | 248 | 21 | 20 | |
| 175 | AGACCATTAACTGAGTCCCAAAT | AGCAGCTAAAACATTGGTCTCT | 39.13 | 40.909 | 57.479 | 57.974 | 58 | 232 | 23 | 22 | |
| 176 | GTTACAGAGCTTGAGAGACCATT | TGGTCTCTTTATTCTGAGCCC | 43.478 | 47.619 | 58.173 | 57.086 | 43 | 218 | 23 | 21 | |
| 121 | GCAGACTTTAGAAGCTTTGAACT | GTCTCTTTATTCTGAGCCCAACT | 39.13 | 43.478 | 57.319 | 58.162 | 96 | 216 | 23 | 23 | |