Aliases circ-9563 Scientific name Ovis aries
Organism Sheep Genome Locus chr8:78516760-78526356:-
CircRNA type intron Gene ID n/a
Gene Name TFB1M Detection pipeline n/a
Sample Type Pitutary gland
Method for Estimation Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer GGACTTGTGTAATGTCAACTTT
Reverse Primer ATGAGAGATTCTACTTTCACCTAT
Pubmed 29170496
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
296 ACAAAAGCCTGACCTGGGAT AAAGACAGTGGGAGGATGGT 50 50 59.22 58.253 13 308 20 20
286 AGCCTGACCTGGGATTGTC CAGTGGGAGGATGGTAAGTGT 57.895 52.381 59.006 59.091 18 303 19 21
282 CCTGGGATTGTCAAGATGTGC AGACAGTGGGAGGATGGTAAG 52.381 52.381 59.253 58.528 25 306 21 21
248 GTCTCTGCAGAAGGACAAAGA GTCCATGGAGATGTCTTGACA 47.619 47.619 57.607 57.383 116 363 21 21
198 CTGTTTGCTTCCAGTAGTAGCT AGTGTCTTTGGGTTGGTTTTCT 45.455 40.909 58.068 58.292 90 287 22 22
265 GGGATTGTCAAGATGTGCTGA TGGTAAGTGTCTTTGGGTTGG 47.619 47.619 58.285 58.061 28 292 21 21
192 GCTGTAAGTCTCTGCAGAAGG TGGGAGGATGGTAAGTGTCTT 52.381 47.619 58.384 58.084 109 300 21 21
195 TCTGCAGAAGGACAAAGAGGA TCTCAAAAGACAGTGGGAGGA 47.619 47.619 58.671 58.31 119 313 21 21
138 TCATCATCTACGAACACCAGGT AGGATGGTAAGTGTCTTTGGGT 45.455 45.455 58.903 59.013 159 296 22 22
172 CTGCAGAAGGACAAAGAGGAATT GGTAAGTGTCTTTGGGTTGGTTT 43.478 43.478 58.922 59.297 120 291 23 23
278 CAGTAGTAGCTGTAAGTCTCTGC GGAAAACTGAGAATTGTCCATGG 47.826 43.478 58.084 57.936 101 378 23 23