Aliases circ-7204 Scientific name Ovis aries
Organism Sheep Genome Locus chr3:85319893-85322066:-
CircRNA type exon Gene ID n/a
Gene Name ENSOARG00000016346 Detection pipeline n/a
Sample Type Pitutary gland
Method for Estimation Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer GATGACCTGGACGAAGGAGAA
Reverse Primer GAGGACGACACCGTTGAGC
Pubmed 29170496
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
354 TGCATCACTCCATTTGCCAG CAGCTCTAAGTCTGCCTCCA 50 55 58.821 58.81 19 372 20 20
309 CATCACTCCATTTGCCAGTCT GTGGAAGAAGTGACCCCAGG 47.619 60 58.28 59.963 21 329 21 20
283 GGTATGGAGGAGGAAAGAGGA AAGACTTCAGGCCGTGGAAG 52.381 55 57.952 59.965 60 342 21 20
218 GTTGCCTCTTCTTGGATTCCT TAACAGCAAGACTTCAGGCC 47.619 50 57.921 57.815 132 349 21 20
250 TGGCCAATTCCTTTCTTCCT CTTCAGGCCGTGGAAGAAGT 45 55 57.001 59.965 89 338 20 20
201 GGCCAATTCCTTTCTTCCTTGT CTCACTGAGGCCACATTTCT 45.455 50 59.1 57.514 90 290 22 20
307 ACTCCATTTGCCAGTCTAAAACA CCGTGGAAGAAGTGACCCC 39.13 63.158 58.53 60.004 25 331 23 19
312 TCACTCCATTTGCCAGTCTAAA AGGCCGTGGAAGAAGTGAC 40.909 57.895 57.634 59.628 23 334 22 19
232 ACTGGTCAGATTCTTAGCAGTTG GCAAGACTTCAGGCCGTG 43.478 61.111 58.426 58.742 113 344 23 18
284 CCTTTCTTCCTTGTTACTGGTCA TGAACCTCACAGCTCTAAGTCT 43.478 45.455 58.285 58.499 98 381 23 22
311 GTATGGAGGAGGAAAGAGGAAAA AGCTCTAAGTCTGCCTCCATTA 43.478 45.455 57.563 58.352 61 371 23 22
184 TCTTCCTTGTTACTGGTCAGATT TGAGGCCACATTTCTATACTTCA 39.13 39.13 57.174 57.308 102 285 23 23
185 TCCTTGTTACTGGTCAGATTCTT TCACTGAGGCCACATTTCTATAC 39.13 43.478 57.174 57.976 105 289 23 23
252 TGTTACTGGTCAGATTCTTAGCA TGCCTCCATTATAACAGCAAGAC 39.13 43.478 57.261 58.801 109 360 23 23