Aliases circ-1995 Scientific name Ovis aries
Organism Sheep Genome Locus chr16:70953505-70963402:-
CircRNA type intron Gene ID n/a
Gene Name ENSOARG00000016113 Detection pipeline n/a
Sample Type Pitutary gland
Method for Estimation Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer GTCATTGTGGCCTTAGTGATT
Reverse Primer ACCTTGAAGAGCCTGTTGAT
Pubmed 29170496
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
239 CGTCCTTGTTCCGATGCTTC GGGGACGAGAACTGTATCCT 55 55 59.278 58.222 40 278 20 20
127 CCAGAATGGGAGGGGATCAG TATCCTTCACCGACACCGTC 60 55 59.23 59.187 138 264 20 20
208 ACCAGCTTCTCCTTCGTCC CGAGAACTGTATCCTTCACCG 57.895 52.381 59.024 58.201 66 273 19 21
303 AGGTCATCTGCACCCTGTC CGTAAGGAGGATGCACACG 57.895 57.895 59.014 58.334 85 387 19 19
127 GAATGGGAGGGGATCAGCTG CTGTATCCTTCACCGACACC 60 55 59.89 57.705 141 267 20 20
304 GAGGTCCTTGAACCCGGAC GTCCTGCTGAACATGCCG 63.158 61.111 59.704 58.819 14 317 19 18
257 TTGAACCCGGACACAGTGG GGGACGAGAACTGTATCCTTCA 57.895 50 59.853 59.241 21 277 19 22
188 GAGCACGGCCTCACTCAC GTGCGGCTCAACGGTGTC 66.667 66.667 60.433 61.713 178 365 18 18
310 TGGCGTCCTTGTTCCGAT TCCTCGACAAGTCCCAGG 55.556 61.111 58.935 57.886 37 346 18 18
169 GGAGGGGATCAGCTGAGC CTGCTGAACATGCCGGGG 66.667 66.667 59.172 61.136 146 314 18 18
109 GATCAGCTGAGCCTGGCG CTTCACCGACACCGTCCCC 66.667 68.421 60.588 62.585 152 260 18 19