Aliases circ-5181 Scientific name Ovis aries
Organism Sheep Genome Locus chr26:27831504-27831799:-
CircRNA type exon Gene ID n/a
Gene Name ENSOARG00000000349 Detection pipeline n/a
Sample Type Pitutary gland
Method for Estimation Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer TCTTGGCTATGTGGAACTGT
Reverse Primer CAATATCAAATGAATCTGTAGAAAA
Pubmed 29170496
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
319 GAAGGCTCTGGTCCTGTGAT AATGGAAGCAGAGCCTCTCC 55 55 59.092 59.454 10 328 20 20
282 CCGGTTGATGGTGAAGAAGC GAAGCAGAGCCTCTCCACC 55 63.158 59.195 59.78 43 324 20 19
387 GGCTCTGGTCCTGTGATGAT GTCGTGGTTGAGTTGGGAAA 55 50 59.164 58.329 13 399 20 20
297 GTAGGTCCGGTTGATGGTGA GCAGAAATGGAAGCAGAGCC 55 55 59.1 59.545 37 333 20 20
262 GGTGAAGAAGCAAGCATCCG TCTCCACCTCCGTCCGTT 55 61.111 59.551 59.883 52 313 20 18
317 GAAGAGGAAGGCTCTGGTCC CAGAGCCTCTCCACCTCC 60 66.667 59.46 58.372 4 320 20 18
293 TGGTCCTGTGATGATGGAGG CCACCTCCGTCCGTTCTT 55 61.111 58.793 58.943 18 310 20 18
352 GGTCCGGTTGATGGTGAAGA TGAGTTGGGAAAATTTGAAGGCA 55 39.13 59.676 59.291 40 391 20 23
348 CATAGAAGAGGAAGGCTCTGG TGAAAGATGGACATGCAGAAATG 52.381 39.13 57.25 57.681 0 347 21 23
267 GAGCCTTCCGGTTTCCCC CATTTGAAAGATGGACATGCAGA 66.667 39.13 60.045 57.681 85 351 18 23
132 TAAAGAACGGACGGGTTTCC AGCCTCTCCACCTCCGTC 50 66.667 57.832 60.363 186 317 20 18
389 AGGAAGGCTCTGGTCCTGT GTGGTTGAGTTGGGAAAATTTGA 57.895 39.13 59.84 57.983 8 396 19 23