Aliases circ1800 Scientific name Zea mays
Organism Maize Genome Locus chr3:213870010-213870626:-
CircRNA type n/a Gene ID n/a
Gene Name GRMZM2G047255 Detection pipeline KNIFE
Sample Type third leaves of B73 V3 stage seedlings
Method for Estimation n/a
Circular RNA status Experimented PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer n/a
Reverse Primer n/a
Pubmed 29155438
Aliases circ1800 Scientific name Zea mays
Organism Maize Genome Locus chr3:213870010-213870626:-
CircRNA type n/a Gene ID n/a
Gene Name GRMZM2G047255 Detection pipeline KNIFE
Sample Type third leaves of B73 V3 stage seedlings
Method for Estimation Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer TCGTCCAGCCACTTGACGAA
Reverse Primer GAGCACGGCGTGTATAACCTC
Pubmed 29155438
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
289 ACTGGCTCGATGAATTTGGC TCCACGGACTGTACCTATGC 50 55 58.902 58.891 102 390 20 20
272 ATTCGCCATTTCCCGCTTTT AACATAGAGCGGGACTTCGT 45 50 59.105 58.816 39 310 20 20
183 GGCTCGATGAATTTGGCCTC CGGTCAAAGAAACGTGCACT 55 50 59.335 59.344 105 287 20 20
197 AGTCTATGCACATTCGCCATT CGATAAGCAGCACGACAAGG 42.857 55 58.077 59.353 28 224 21 20
127 CCTCTGTCTTTTCGTCGGAC AGAAACGTGCACTGTCTCAC 55 50 58.301 58.428 154 280 20 20
169 GGCCTCTGTCTTTTCGTCG GACTGCGCGCAACATAGAG 57.895 57.895 58.554 59.367 152 320 19 19
190 TTGGCGGCCTCTGTCTTTT TCTTCGGCAATCAACAGACTG 52.632 47.619 59.849 58.583 147 336 19 21
155 GTCTTTTCGTCGGACATTTTGC CGCAACATAGAGCGGGACTT 45.455 55 59.029 60.46 159 313 22 20
123 GCTCGATGAATTTGGCCTCTAG CAACCGATAAGCAGCACGAC 50 55 59.195 59.63 106 228 22 20