Aliases circ1976 Scientific name Zea mays
Organism Maize Genome Locus chr6:118009053-118010000:-
CircRNA type n/a Gene ID n/a
Gene Name GRMZM2G431708 Detection pipeline KNIFE
Sample Type third leaves of B73 V3 stage seedlings
Method for Estimation n/a
Circular RNA status Experimented PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer n/a
Reverse Primer n/a
Pubmed 29155438
Aliases circ1976 Scientific name Zea mays
Organism Maize Genome Locus chr6:118009053-118010000:-
CircRNA type n/a Gene ID n/a
Gene Name GRMZM2G431708 Detection pipeline KNIFE
Sample Type third leaves of B73 V3 stage seedlings
Method for Estimation Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer ACTATCAATCCTTCTCACAATCTCC
Reverse Primer GAGAAGGGCGGTTATGCTGT
Pubmed 29155438
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
171 CCCGGGGTCCATTTTCCTTA TGGTGGCTCTAAGAAGTACGA 55 47.619 59.373 58.201 113 283 20 21
115 AGGCCTTGTTATTTGCAGCT TGATTTTGAAGAGATGGTGGCTC 45 43.478 58.071 58.988 183 297 20 23
237 TCAACTGCAAAATTCGGGGC CACTTGGAACTTGGAAGGTTC 50 47.619 59.683 57.01 7 243 20 21
259 GGGCGTTACAAACAAACACA GTGGCTCTAAGAAGTACGAGAC 45 50 57.437 57.981 23 281 20 22
194 GCCTTGTTATTTGCAGCTCCT TCGTTGGGATGAAATTGATTACC 47.619 39.13 59.179 57.172 185 378 21 23
125 CAAACACCAAAACTCAATTAGCC CTAAGGAGCTGCAAATAACAAGG 39.13 43.478 57.095 57.759 84 208 23 23