| Aliases | circ2099 | Scientific name | Zea mays | ||||
| Organism | Maize | Genome Locus | chr5:40379263-40380024:- | ||||
| CircRNA type | n/a | Gene ID | n/a | ||||
| Gene Name | GRMZM2G151195 | Detection pipeline | KNIFE | ||||
| Sample Type | third leaves of B73 V3 stage seedlings | ||||||||
| Method for Estimation | n/a | ||||||||
| Circular RNA status | Experimented | PCR Details | Link to the detailed experimental procedure | ||||||
| Primers (Experimented) | |||||||||
| Primers (Validated) | |||||||||
| Forward Primer | n/a | ||||||||
| Reverse Primer | n/a | ||||||||
| Aliases | circ2099 | Scientific name | Zea mays | ||||
| Organism | Maize | Genome Locus | chr5:40379263-40380024:- | ||||
| CircRNA type | n/a | Gene ID | n/a | ||||
| Gene Name | GRMZM2G151195 | Detection pipeline | KNIFE | ||||
| Sample Type | third leaves of B73 V3 stage seedlings | ||||||||
| Method for Estimation | Quantitative PCR | ||||||||
| Circular RNA status | Validated | PCR Details | Link to the detailed experimental procedure | ||||||
| Primers (Experimented) | |||||||||
| Primers (Validated) | |||||||||
| Forward Primer | AGAACGTGAACGTGAGATGCTTT | ||||||||
| Reverse Primer | AGCAGAGACTTCCCATACATGACTA | ||||||||
| Suggested primers | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Product size | Left primer | Right primer | Left GC | Right GC | Left TM | Right TM | Left pos | Right pos | Left size | Right size | |
| 197 | CTTGCCGGATTTCTACGAGC | TCTTGCAAACCCACTACATCT | 55 | 42.857 | 59.069 | 57.212 | 188 | 384 | 20 | 21 | |
| 225 | GTGCTTGCCCCGAACATATT | TTGCAAACCCACTACATCTATTG | 50 | 39.13 | 58.897 | 57.104 | 158 | 382 | 20 | 23 | |