Aliases circ2408 Scientific name Zea mays
Organism Maize Genome Locus chr2:132713831-132714323:+
CircRNA type n/a Gene ID n/a
Gene Name GRMZM2G416491 Detection pipeline KNIFE
Sample Type third leaves of B73 V3 stage seedlings
Method for Estimation n/a
Circular RNA status Experimented PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer n/a
Reverse Primer n/a
Aliases circ2408 Scientific name Zea mays
Organism Maize Genome Locus chr2:132713831-132714323:+
CircRNA type n/a Gene ID n/a
Gene Name GRMZM2G416491 Detection pipeline KNIFE
Sample Type third leaves of B73 V3 stage seedlings
Method for Estimation Quantitative PCR
Circular RNA status Validated PCR Details Link to the detailed experimental procedure
Primers (Experimented)
Primers (Validated)
Forward Primer GGCAGAAATCTAACGAAGTTCATTT
Reverse Primer CAACACATCAGTATAGCGGTTTCTT
Suggested primers
Product size Left primer Right primer Left GC Right GC Left TM Right TM Left pos Right pos Left size Right size
127 ACTTCGAACGTCAGGGTCTC CAGTGCTCGTCGACTTTCTC 55 55 59.403 58.668 107 233 20 20
159 CTTCGGAAGTTGGAGGGTGT CGACACCACCAAGCAGATC 55 57.895 59.603 58.54 128 286 20 19
239 GGCCATGAACTTCGCGTAG CCACCAAGCAGATCCGGAT 57.895 57.895 58.993 59.471 43 281 19 19
241 CCACTTCGAACGTCAGGGT CATGCCGGCAACTTCGAG 57.895 61.111 59.634 59.213 105 345 19 18
215 GCATCTTGAGCTTCAGGTAGG CGACTTTCTCCGCGCAAAC 52.381 57.895 58.441 60.155 9 223 21 19
188 GACGGCCATGAACTTCGC TCGTCGACTTTCTCCGCG 61.111 61.111 59.215 59.825 40 227 18 18
157 CGGCGTGGTAGGTTCCTC GCAGTGCTCGTCGACTTT 66.667 55.556 59.816 58.062 78 234 18 18